Hello, Would anyone be kind enough to suggest some techniques for analyzing adherent cell types by flow? Specifically, we are looking at a human cell line of promyelocytes, which when treated with phorbol esters differentiate into monocytes & macrophages. We are attempting to analyze changes in CD 14 expression. We are having problems with the cells adhering to one another after trypsin treatement to lift the cells from the plates. many thanks, Janet
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