> "Esper G. Kallás" wrote: > > > Using Annexin V FITC (PharMingen 65874X), we found that all monocytes > stain in the FL1 channel, around 10^3. We also recently completed a study in which we observed what appeared to be connstitutive binding of annexin to monocytes. The system used annexin biotin followed by steptavidin APC in combination with various monoclonal antiboides to distinguish cell populaitons. our objective was to measure lymphocyte apoptosis, so we used gating strategies to eliminate the monos and did not pursue the reason for this. Regards, Tom ***************************************************************************** Thomas W. Mc Closkey, Ph. D. Director of Flow Cytometry, North Shore University Hospital Assistant Professor of Pediatrics, New York University School of Medicine Boas Marks Biomedical Research Center, 350 Community Drive Manhasset, Long Island, New York 11030 ph: 516-562-4844 [office], 516-562-1135/4641 [lab] fax: 516-562-2866 *****************************************************************************
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:55:56 EST