Dear Flowers, Hello and best wishes. We want to develop an assay for the detection of IL4 intracellularly in an appropriate human T cell line (Th2 profile of cytokine expressing cell line). At present we are using a Jurkat cell line and are not able to detect IL4 intracellularly even though PBMC show it. There are some doubts that I have and would be grateful for any help regarding them. 1. There are a couple of Jurkat derived cell lines but most of them are stated to produce IL2 ( as mentioned in ATCC catalogue), has any one used these cell lines for detection of IL4 after stimulation with Ionomycin and PMA? 2. What are the cell surface markers that define these cells? 3. Are there any other human T cell lines that produce IL4 upon stimulation with Ca2+ ionophores and PMA? Thanking you in advance for your responses. Regards Rana Nagarkatti Molecular Immunology Div. Center for Biochemical Technology Mall Road, Delhi, India 91-110007 ____________________________________________________________________ Get free email and a permanent address at http://www.netaddress.com/?N=1
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