We have had good luck using a trition permeabilization method for intranuclear staining. It seems that saponin is not strong enought to do this: basically (for a million cells) we fix the cells in 2% FRESH paraformaldehyde for 15-30 min at 4 degrees/spin down/resuspend pellet in in 0.1% triton x-100 diluted in PBS for 3-6 minutes/wash and then stain... hope this helps.. good luck! jonni Jonni S. Moore, Ph.D. Director of Clinical and Research Flow Cytometry University of Pennsylvania School of Medicine 203 John Morgan Bldg. Philadelphia, PA 19104-6082 Phone: 215-898-6853
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