Europium chelates light emission half life is of the order of a hundred msec. Thus, they work fine on fluorimeters, but are unsuitable for significant signal collection in flow. Saverio Alberti Head, Lab. of Experimental Oncology Department of Cell Biology and Oncology Consorzio Mario Negri Sud 66030 Santa Maria Imbaro (Chieti), Italy Phone: (39-0872) 570.293 FAX: (39-0872) 570.412 E-mail: alberti@cmns.mnegri.it On Mon, 26 Jun 2000, alan saluk wrote: > > Flowers-- > > I had a researcher come into the lab with a Eu-chelate as the > fluorochrome. He came without literature and said it had an excitation > peak around 345nm (I used the relatively weak 351nm line from an > Enterprise) and a very narrow emission at 615nm (I used a 610/20 to > collect). I was unable to see any kind of signal although his controls > using a FITC-conjugate worked fine. I was a little suspicious when he > said it was "quantumly inefficient" and he had never seen it tried on a > flow cytometer (I'm betting it might need a little more time in the > beam). Do any of the gurus out there have any wisdom to share. > > Al > > asaluk@scripps.edu > >
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