Re: dsRNA dye?

From: Gretchen Lawler (GRETCHEN@flowcyt.cyto.purdue.edu)
Date: Thu Jun 22 2000 - 08:03:41 EST


David McFarland asks:

>Is there such a beast as a dsRNA specific dye or at least one that can be
>distinguished spectrally when it binds dsRNA?  How about all this and cell
>permeant?  Am I dreaming or is this within reach?

David,
In addition to the suggestions by Howard Shapiro, see also Current
Protocols in Cytometry, unit 7.3: Differential Staining of DNA and
RNA, by Z. Darzynkiewicz and G. Juan.

Gretchen Lawler



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