It does look as a T lymphoproliferation, ATCL (adult T cell lymphoma/leukemia) on the first place (other T malignancies should be CD7+). If I understand correctly, the small population of 1.5% stains with most antibodies and it may represent a subpopulation of dying or damaged cells. HTLV1 is the next potentially helpful screening as all cases with ATCL should be HTLV1+ (it is probably pending already). TCR genes should be rearranged. If HTLV is neg, other T-malignancies should be considered. Best regards, Ondrej Hrusak At 0:17 -0800 16.11.1999, JUAN LUIS CASTILLO NAVARRETE wrote: >I have a sample from a woman, 59 years, walking, with the diagnosis of >lymphoma. >Dont have adenopaties, lttle splenomegaly, with gladder stone, total >bilirrubin 4.5 mg/dl, GOT, GPT a and Alcaline fosfatase high. >The haematolgical parameters are : WBC "113.000", Plt 53.000, Ht 39.6%, >Hb 13.7% . 80% of elements of size regular, convolute nuclei, with one >ocassionaly nucleoli. Agranular and basophil citopllasm. >The CF show a population of 87 % of cells that have a SSC, FSC and CD45 >normal. Also are CD19(-), CD20(-), CD34(-), CD33(-), CD13 (-), CD14 (-), >CD10 (-), Tdt (-), MPO (-), and CD3(+++), CD4 (+++), CD5 (+++), CD2(-), >CD7(-), CD8(-), HLA-DR (++) (58 %), NK(-). >This population, CD3(+++), have a little porcentaje that are very brigth >(1.51%) and are CD2(+++), CD7(+++), CD5(+++), CD4(++)(52.5%), >CD8(++)(44.09), ratio CD4/CD8=1.1, and HLA-DR(+)(29.3%). The big >population that are CD3(+++)dim (really more than dim) are CD4(+++), >CD8(-), CD2(-), CD7 (-), CD5(+++) and HLA-DR (++)(57.6%). > >Opinions? > >Thanks >-- >JUAN LUIS CASTILLO N. >CITOMETRIA DE FLUJO >HOSPITAL DEL TRABAJADOR >CARDENIO AVELLO 36 >CONCEPCION >CHILE >PHONE: 56-41-402138 >FAX: 56-41-402117 >EMAIL: axelyoyi@entelchile.net >http://oncoinmun.bizland.com/ Ondrej Hrusak, M.D., Ph.D. Institute of Immunology Ondrej.Hrusak@lfmotol.cuni.cz fax +4202 2443 5962 http://www.lf2.cuni.cz/clip/ phone +4202 2443 5969, +4202 2443 2084 postal address: V uvalu 84 150 06 Praha 5 Czech Republic
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