Dear Flow friends, we have been organizing the virtual campus and as part
of such effort we are aiming to produce an e-book for flow cytometry. I am
asking for potential partners to contribute in this project. We have
develop a tentative list of topics that are listed down. If you feel that
you can help and participate, please send me a note and we can forward
additional details. As is listed, an e-book in flow cytometry target
students and scientist fond or in need of this information. We have a
unique opportunity to go in the web and contribute to our effort of the
virtual campus. This is an project that should be completed by the end of
the summer 2000. We have plenty of gigabits for graphics and diagrams.
Suggestions and additional chapters are welcome. As soon as each chapter is
finished, we attempt to put it on-line. I hope to have as many partners as
is possible, we have an inclusive tent and contributions from not
traditional places are welcome. We hope to have contributions from Asia,
Africa, Australia, South America and of course Europe and America.
1-Introduction
Theoretical introduction to the field of Cytometry
What is Flow Cytometry and its importance?
2-Instruments for Flow Cytometry
FACSCalibur
FACSVantage
Beckman Coulter
MoFlo® Cytometers
Other non-commercial produced flow cytometers. The "HiReCS" system
3-Flow cytometric data analysis
Gating
Histograms and setting markers
Dot Plot
Contour plots/Density plots
4-Molecular Cytometry
What is "Molecular Cytometry"?
Flow cytometry/cell sorting for cell Micro-manipulation
FISH ("fluorescence in-situ hybridization") in cytometry
PCR in molecular cytometry
Image and Confocal microscopy analysis
References
5-Handling of samples
Biosafety
Staining
"Fluorescein labeling of proteins
Phycoerythrin conjugation protocol
References
6-Surface analysis and immunophenotyping by multicolor analysis
Immunophenotyping of Leukemia and Lymphoma samples
General References for Immunophenotyping
Other applications of surface analysis and immunophenotyping
Reticulocyte analysis
Platelelet-associated Immunoglobuling (PaIg)
Reference
CD4 T cell enumeration and Immunophenotyping for diagnosis of
Immunodeficiency or treatment follow up.
References
Enumeration of CD34+ cells (Stem cells)
References
Immunophenotyping for T cells CD45 RA/RO
References
7-Intracellular antigens
Cytokines and study of Viral antigens
8-DNA Analysis
DNA measurement and Cell Cycle Analysis
Flow cytometry measurement of cellular DNA content
The analysis of the cell cycle
Approach to discriminate Doublets or cellular aggregates
Software for cell cycle analysis
Bivariate BrdU/DNA
DNA Content Analysis
References
9-Special applications of flow cytometry
10-Software for data analysis-CellQuest’/FLOWjo’/WinDi’
CellQuest
FLOWjo’
WinDi
11-Protocols
12- Commercial Resources for Flow Cytometry on the Web
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(o o)
________________________________oOo__(_)__oOo_________________________________
___/\_ | Rafael Nunez mailto:rafaeln@vetvir.unizh.ch
/ o \/| | University Inst.for Virology http://www.vetvir.unizh.ch/
/ _| | Winterthurerstr. 266a Telephone: (+41) 1 6358710
/_/\__/-\/ | 8057 Zurich SWITZERLAND Faximile : (+41) 1 6358911
______________________________________________________________________________
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