Marylou Zuzarte writes- >i would like to know if anyone has tried measuring membrane potential on >isolated mitochondria on the FAcs and whether it has been successful. People have looked at isolated mitochondria with rhodamine 123 and probably other dyes such as JC-1; these, however, generally provide a relatively crude estimate rather than a calibrated value of membrane potential. A more accurate measurement could probably be obtained using the ratiometric technique with DiOC2(3) developed in my lab for bacteria by Dave Novo (Cytometry 35:55-63, 1999); this probably won't work for mitochondria in situ but should for isolated mitochondria. -Howard -- End --
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