To whom it may concern! I am a student at Dept. Tropical Medicine, Tulane University. I am detecting ICAM-1/CD54 from rhesus monkey brain endothelial cells. As my positive control, human brain endothelial cells. I have detected ICAM-1 in human brain endothelial cells (constitutive and upregulated by TNF), but I failed to detect ICAM-1 in rhesus monkeys. The MAbs that I have used: 84H10 (Immunotech) and LB-2 clone (BD). LB-2 clone according to BD will cross-react to ICAM-1 of rhesus monkey (based on a web-site at Univ. Florida). So, my questions: 1. what should I do so that I could detect ICAM-1 from rhesus monkey? 2. is trypsinization will destroy ICAM1 from rhesus monkeys? (my positive control is trypsinized and works well) 3. I have tried to detach my cells with EDTA 1 mM in PBS and it did not work. Will Sigma Cell Detachment Solution will work? Or, if somebody knows how to detach this kind of sticky cells (without scrapping and trypsin) and still preserve ICAM-1? 4. is there somebody who has work with ICAM-1 in rhesus monkey? I wonder if the researcher(s) who put the information on U Florida website could share the procedure! Thanks. Sukwan Handali
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:55:53 EST