We are having problems with biotinylating purified monoclonals that we have stored in 50% glycerol in PBS. We dialyze vs. 0.05 M Borate at pH 8.6 and then use the Amersham Life Sciences Biotinylation Kit. We do this routinely with purchased monoclonals, usually lyophilized in PBS. Has anyone else experienced problems with glycerol or do you have any suggestions? Thanks, Kathi Kellar, PhD National Center for Infectious Diseases CDC 1600 Clifton Rd, NE Atlanta, GA 30333 404-639-1419 FAX 404-639-3129 klk1@cdc.gov
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