On Fri, 2 Jun 2000, Len Brown wrote: > > Dear All > > We are attempting to set up 4 colour assays on our FACS Vantage (NOT SE > upgraded) using APC on a HeNe laser delivering about 30 mW. Abs are from BD. > > 1) Should we expect to see the APC signal in the FL3 channel (optimised for > PECY5) of our 488 nm line? ie. Is APC excited at all by the 488 nm laser? > yes, APC is excited just a bit by 488n,m light. If you want to separate APC single positive cells or APC dim cells then use PE-TxRed since you cannot compensate; the reciprocal Cy5 in APC can only be done with the OmniComp add-on or the new digital system when released. Dave > 2) Is anybody using BD's CD11c-APC mAb on a similar system, if so how does > the signal compare with PE. > > Any help or hints on setting up 4 colour on a Vantage with 488 and 635 nm > laser lines would be most appreciated. > > Len Brown >
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