Have you tried using DNase? On human cells it works very well, perhaps it would on mouse cells, too. Marj Williams Lehigh Valley Hospital HLA Lab. ------------------( Forwarded letter 1 follows )-------------------- Date: Thu, 25 May 2000 09:45:33 +0100 To: cyto-inbox From: a.pizzey@ucl.ac.uk Sender: owner-cyto-sendout@flowcyt.cyto.purdue.edu Subject: Re:Dead Cell Removal > >What is the most efficient method for removing dead cells from a single cell >suspension prepared from mouse spleens? > >Thanks in advance. > >Delynn Moss >Division of Parasitic Diseases >Centers for Disease Control > > Greetings Delynn, You might have a look at Miltenyi's website, they have an Apoptotic cell isolation kit which will do the trick. http://195.227.58.203/produkte/humreag/apoptotic.htm Regards, Arnold _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 020-7679-6234 Fax: +44 020-7679-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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