Mark et al.,
I looked into the various anti-BrdU clones a few years ago. There are several available beside the Florida/Gratzner's clone (U Cal, U Minn, private, etc.). Unfortunately I do not remember all of the details; but, just out of curiosity, I did look up the information on the Gratzner clone. I think the patent of interest actually issued in July of 1985, so I do not think it will be in the public domain until July 2002. Below is a cut and paste job from the IBM patent website showing claim 1 listing ATCC deposit info.
I hope this helps.
Ty Lee
US4529700 07/16/1985 Hybridoma cells secreting a monoclonal antibody
specific for 5-bromo and 5-iodoeoxyuridine and reagents for measuring
cellular proliferation
Claim: I claim:
1. A hybridoma which is deposited at the American Type Culture
Collection under ATCC accession number HB-8150 and which is composed of a
hybrid cell resulting from the fusion of a murine plasmacytoma cell from
plasmacytoma cell line SP 2/O Ag 15 with an antibody-producing mouse spleen
cell from a mouse immunized with the conjugate of 5-bromouridine or
5-iodouridine and an albumin carrier molecule, said cell producing
antibodies specific for 5-bromodeoxyuridine or 5-iododeoxyuridine.
-----Original Message-----
From: Mark Shlomchik <mark.shlomchik@yale.edu>
To: cyto-inbox
Date: Tuesday, May 23, 2000 1:59 PM
Subject: anti-BrdU clone B44
>In 1982 Dr. H. Gratzner published a landmark paper on the detection
>of BrdU in DNA by Flow using a mAb he made called B44. This is in
>fact the clone that is sold by BD for $420 for 100 tests (about 50ug)
>or over $8,000 per mg. This work was done at a public university
>(Univ. of Miami) and funded by the NCI. Therefore, 18 years later
>this clone should be available and in the public domain.
>
>My question: does anybody have this cell line or know where or how it
>can be obtained for research work?
>
>I would be interested in any clues or if anyone knew any of Dr.
>Gratzner's colleagues (Dr. G. has since passed away).
>
>Thanks,
>
>Mark Shlomchik, MD, PhD
>Associate Professor of Laboratory Medicine
>and Immunobiology
>Yale University School of Medicine
>
>203-688-2089
>203-688-2748 (fax)
>mark.shlomchik@yale.edu
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