greetings all, I'm revisiting the topic of CD45 dim cells once again: we have had quite a few clinical samples whose purities were muchly affected by the presence of CD45 dim cells in the CD45/14 tube by which we set our lymphocyte gate. The purity correction is much different in simulset (automatically excludes dims) or cellquest (manually includes). These differences can be pretty high. I am going to include a review of with/without on my webpage at http://www.cd4cd8.com/cd45dims.html this evening (Tues. May 23) so you can see the differences in B, Th, Tc and NK cells (a lot of you already know what I'm talking about). I wonder if there is any new agreements in labs who do clinical flow and have to worry about what to report out? You all know how crucial absolute CD4 counts are in HIV patients. regards, Maciej __________________________________________________ Do You Yahoo!? Send instant messages & get email alerts with Yahoo! Messenger. http://im.yahoo.com/
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