Hello to all,
I have a user who would like to look at Ca+2 levels. I have never run
this before and would like to know what others think is the best
reagent for this measurement. We have a UV laser (Coherent Innova
300) on our MoFlo so we can run Indo1. In my reading, Indo1 seems
like the dye of choice since using the ratio of the 2 emissions for
bound and unbound Ca+2 eliminates problems with loading differences
and size differences of a population of cells. What do you
experienced cytometrists think? Thanks for your input.
Lucy Brown
Beckman Research Institute/City of Hope
626-359-8111 X3306
lbrown@coh.org
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