Hello Flow-ers, Our lab is considering using BD's FACS Lysing Solution for our regular surface staining panel (CD3, 4, 8, 20 and 16) for use with rhesus samples. I would greatly appreciate any information concerning the performance of this method. I have been told that this method doesn't work so well with intracellular staining. We also are curious to know if anyone has used this method with CD 62L or the naive/memory markers CD45 RO/RA. Thank you in advance, Dagna Sheerar Immunology and Virology Core Laboratory Wisconsin Regional Primate Research Center University of Wisconsin 1220 Capitol Court Bldg #2, Rm #215 Madison, Wi 53715 608 265 3796 dagnas@primate.wisc.edu
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