Hi everyone, itīs me again! I am going to start doing intracytoplasmic stainning of gamma IFN in human PBLs using the Pharmingen kit (Cytofix/Cytoperm with golgi plug). Could you please send me some useful tips on this technique (like for example wich kind of stimulation to use as positive control, period of stimulation, etc..) Thanks so much Jose Miguel ________________________________________________________________________ Get Your Private, Free E-mail from MSN Hotmail at http://www.hotmail.com
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