CFSE staining - Large Numbers of PBL

From: Barsky, Lora (LBarsky@chla.usc.edu)
Date: Tue May 16 2000 - 12:10:59 EST


Hi,

I'm posting this on behalf of a graduate student here at Children's.  From
what I know of his project, he is working with a murine model and has done
murine to murine BMT in the past.  If you can help him, please e-mail him
directly at dudl@usc.edu or post to the mailing list.

Thanks,  Lora Barsky, CHLA

Eric's Question:
	I've been trying to find a way to stain large numbers  (20-50
million) of murine peripheral blood lymphocytes to study their survival and
proliferation.	Has anyone successfully labelled this many cells in such a
way as to be able to track their survival or proliferation in vivo?  Any
help would be appreciated.

Eric Dudl
Graduate Student
BMT/Research Immunology
Children's Hospital - Los Angeles



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