Dear Akos, Even when they were on their own, they were very expensive. What I do is sort exact number (usually 50.000) of alignment beads using CloneCyt or just Counter mode in a FACS tube and then add the sample in. That's how I check numbers of cells sorted and double check the recoveries. This correlates very well with TruCount tubes and more/less with what users count on microscope (same as with TruCount). Problem is that it has to be made fresh and that there could be a few doublets biasing towards lower calculated number. It virtually doesn't cost anything, I just hope you can find a sorter nearby. Sasha. ************ Dr Sasha Sreckovic Dept Path & Micro University of Bristol University Walk Bristol, BS8 1TD, UK Sasa.Sreckovic@bristol.ac.uk +44-(0)117-928-8606 ************ ---------- >From: Akos_Szilvasi@biogen.com >To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu> >Subject: TruCount substitute >Date: Tue, May 9, 2000, 14:21 > > > > > Dear Flowers, > > BD does not make the TruCount tubes available without buying it with their kits > (that we do not need). Does anyone know a similar product for cell counting on a > flow cytometer? > > Thanks, > > Akos Szilvasi > Biogen, Cambridge, MA > > >
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