Does anyone have experience or know of antibodies that can be used to identify and follow erythrocyte maturation in rat bone marrow? The Pharmagen rat CD chart shows only CD71 (transferrin receptor) as being relatively specific to the erythroid series, but it is expressed on lymphocytes as well. Rat bone marrow is composed of up to 60% B lymphocytes which are in the same FS and SS location as the maturing erythroid series. In our hands, CD71 has not shown good separation between lymphocytes and erythoid cells. We would like to label erythroid series specifically and follow cell cycle effects in that population. Thanks in advance for any help. Carol W. Johnson DVM PhD Pharmacia Kalamazoo MI
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