Maris Handley recently inquired about measuring DNA content with 7-AAD in conjunction with multicolor immunofluorescence measurements-the latest word on this is: Schmid I, Cole SW, Zack JA, Giorgi JV: Measurement of lymphocyte subset proliferation by three-color immunofluorescence and DNA flow cytometry. J Immunol Methods 235:121-131, 2000. -Howard
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