"Beverly E. Barton" wrote: > > To the Cytometry List: > > I have successfully used GIBCO's Enzyme-Free Cell Dissociation Buffer > for many adherent cell types with the annexin V/PI assay for apoptosis. > The one cell type I was unsuccessful with is HUVEC. For these cells, > since I have to trypsinize the cells for the maximum viability, I use > Molecular Probe's JC-1 mitochondrial stain to measure apoptosis by > mitochondrial membrane depolarization. This has worked very nicely. Beverly: What do you know of its composition? Is it somewhat like the "Versene" solution that is basicall PBS w/o Ca2+/Mg2+ but may have anywhere between 0.5-5 mM EDTA? The latter solution works well in my hands @ 5mM EDTA but as one might guess - it doesn't do well for those cells that are moderately to very well attached. Many thanks, David =========== David L. Haviland, Ph.D., Asst. Prof. Immunology University of Texas - Houston, H.S.C. Institute of Molecular Medicine, R907 2121 W. Holcombe Blvd., Houston, TX 77030 713.500.2413-Voice//713.500.2424-FAX ----------------- If everything seems to be going so well, you have obviously overlooked something. ==========
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