Hi: I need your input. What are the best parameters to use when acquiring samples for FITC-annexinV and PI? In the past, our parameters were FL2-H vs FL1-H. My colleague prefers using FL3-H vs FL1-H. What are the pros and cons for both ways? We are using myeloma cell lines. My second question: can you perform annexin V binding on adherent cell lines? Thanks in advance, Rena
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