Hi folks, I was just approached by a student who is looking at Mycoplasma pneumoniae, and he's got an interesting question. He has a mutant that seems to have an altered chromatin condensation, which he has been looking at using image analysis, but he would like to use flow. His approach is to stain the DNA w/ DAPI, and the membrane with another dye, and then analyze the area associated with each color. The ratio of nucleoid area to total cellular area differs btwn wild type and mutant. He considers the microscopy a pain, and would like to use flow, but this type of spatial analysis is the type of thing that imaging is perfect for. For one thing, these things are tiny, and bad about clumping. Has anyone had any success with M. pneumoniae or relatives in a flow system?? Should I be giving this guy a pitch for a laser scanning system? Any suggestions welcomed, Steve +++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Steve G. Hilliard (706) 542-9474 University of Georgia Cell Analysis Facility flowman@uga.edu http://floweb.cb.uga.edu/
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