>Dear Flow Group, > > >I have seen several publications lately that show BrdU cell cycle >analysis, but show >both PI and FITC channels on a linear scale. I generally look at FITC on >a log scale. >Does this affect results in any way? > > >Thanks for any comments. If BrdUrd-labeled fluorescence is measured with logarithmic amplification the effect is to make the marginal distribution approximately normal. This (in my opinion) makes it much easier to discriminate labeled cells compared with linear amplification. Others I know disagree. Best wishes, Nick Nicholas Terry, M.A., Ph.D., Experimental Radiation Oncology - 066, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Blvd., Houston, Texas 77030. 'Phone: 713-792-3424, 'Fax: 713-794-5369. http://drad52.mdacc.tmc.edu. ntflow@odin.mdacc.tmc.edu.
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