micelle or liposome sorting

From: J.Brian Windsor (b.windsor@mail.utexas.edu)
Date: Thu Apr 27 2000 - 11:03:13 EST


Does anyone have any information on sorting micelles, liposomes, or
liposome-like compartments? I would like to do in vitro
transcription/translation in some sort of compartment (about 1.5 micron
diameter) and directly measure properties using flow cytometry (NOT fuse
these liposomes to cells, then sort). I found a protocol for doing this
sort of transcription/translation in a water/mineral oil emulsion, but I'm
pretty sure that I can't run mineral oil through the cytometer.

Any ideas? Thanks-


Brian Windsor
Alan Lloyd Lab    ICMB
The University of Texas at Austin
MBB 1.448B    mail code: A4800
Austin, Texas  78713
(512) 471-3553
fax: (512) 232-3432
b.windsor@mail.utexas.edu



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