On Wed, 19 Apr 2000, Joy Mundy wrote: > Does anybody have a good diagram they can reference or send as an > attachement that shows the spectral emission overlap for the following > fluorchromes; > FITC, PE, ECD and PCY5 Hi Joy, Bio-Rad have, on their website, an excellent fluorochrome database that allows you to plot emission and excitation spectra for a wide range of fluorochromes: http://fluorescence.bio-rad.com/ You will probably need to input ECD as Texas Red and PECy5 as Cy5 but it should be able to give you an idea of the emissions and therfore the overlap. Other useful sites include: Molecular Probes: http://www.probes.com/ Jerusalem College of Technology: http://optics.jct.ac.il/~aryeh/Spectra/GIFs/ Derek ************************************************************************ Derek Davies Voice: (44) 0207 269 3394 FACS Laboratory, FAX: (44) 0207 269 3100 Imperial Cancer Research Fund, e_mail: derek.davies@icrf.icnet.uk London, UK mobile: 07790 604112 Web Page: http://www.icnet.uk/axp/facs/davies/index.html In tenebris lux *************************************************************************
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