At 06:56 PM 4/18/00 -0500, Kevin Waddick wrote:
>
> Is it really necessary to wash cells between incubation with a
>biotinylated antibody and adding streptavidin that is linked to a
>fluorochrome?
No. See:
Dale GL.
Rapid production of quasi-stable antibody-phycoerythrin conjugates for
use in flow cytometry.
Cytometry, 1998 Dec 1, 33(4):482-6.
> That is, doesn't it work as well if the two are added
>together, thereby saving a step? I suppose that I could test this
>myself, however I would consider limited attempts by me to be anecdotal.
>Others out there may have done actual studies of this and might even
>know of tricks to make it work -- assuming that it is not as simple as I
>described. That's what I'm hoping, anyway!
>
>Kevin G. Waddick, Ph.D.
>Parker Hughes Institute
>2657 Patton Road
>St. Paul, MN 55113
---
Dennis
Dennis J. Young
Mail:<<mailto:djyoung@ucsd.edu>>
WWW:<<http://cancer.ucsd.edu/SResources/flow.htm>>
Flow Cytometry Core Facility
University of California, San Diego
Internal Medicine Group, Bldg #4, Rm 126
9500 Gilman Drive La Jolla CA 92093-0671
Telephone:(858) 822-0407
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