When we have analyzed PFA fixed PBMC, the basophils (IgE+, lin-) cells were somewhat between the lymphs and moncytes on the FSC/SSC plot. I have not read it, but Larry Schwartz has a paper purifying basophils by sorting on their scatter characteristics [Kepley et. al, J. Imm Methods 175:1 (1990)]. Calman > ---------- > From: Alfredo Vannacci > Sent: Thursday, April 13, 2000 6:15 AM > To: Cytometry Mailing List > > Dear Flowers, > I would like to know if someone has experience in gating human basophils > according to their FS/SS pattern. > I read some articles in wich was described a gate on lymphocyte region. > Any comment would be apreciated. > > Dr Alfredo Vannacci, MD > > Università degli Studi di Firenze > Dipartimento di Farmacologia Preclinica e Clinica "Mario Aiazzi Mancini" > Unità Operativa di Tossicologia Medica e Medicina delle > Farmacotossicodipendenze > Viale Pieraccini 6, 50139, Firenze, Italia > > e-mail vannacci@pharm.unifi.it > >
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