In a message dated 4/3/00 4:53:31 PM, rr5845@hotmail.com writes: << Hi We use the elisa technique succesfully in our laboratories to detect phage bound to the leukemic cells. We use the monoclonal mouse anti-M13 antibody conjugated with HRP to detect the bound phage. But our repeated attepts to detect bound phage by flow has failed. I have used Mouse anti-M13 unconjugated primary antibody followed by using a anti mouse goat secondary which is conjugated to FITC. A couple of papers have shown bound phage detection by using a sheep anti-M13 antibody followed by secoundary conjugated with FITC with success, But the company Parmacia stopped production of this antibody. The company has not tried out the mouse anti M13 primary to check phage bound to cell by flow. So i would be very grateful if anybody could give me information on protocols tried out or to use or information of antibodies that may be used for detection of the bound phage by flow. I have also tried out anti HRP antibody against the anti-M13 HRP primary that we use for the ELISA but still could not show the detection of phage bound to the cells. Thanks Raj ____ >> Raj, Exalpha Biologicals, Inc. (800. 395. 1137 or 617. 445. 6463) has an anti M13 antibody that is biotin conjugated. We haven't tested it in flow yet but it may be worth a try - it's not the Pharmacia clone so who knows. Regards, John
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