I've had the second beta instrument that was shipped in October of last year. It's been in a multi-user facility since then. Performance: boringly stable Alignment: there is none (good since it's not simple) Optics: we only swap bandpass filters in front of the PMTs Ease of use: if someone can use CellQuest, they can run the instrument But, for complex things (calcium flux with indo-1, DNA (DAPI or Hoechst) with three or four surface markers, it's important that an experience person setup the application and make sure that the configuration is correct and the detector setup and amplifications are appropriate. After that, small changes can usually be done by the user. Quite naive users are able to run dual laser, multiparameter experiments and get good results. Most applications can be done as comes out of the box. If you'll be changing dichroics, new holders would be needed (I've made some modifications). If the initial alignment is good, then positioning the dichroic has limited degrees of freedom as far as realignment is concerned. Dave ********************************** David M. Coder, Ph.D. Director, Cell Analysis Facility Dept. of Immunology Univ. of Washington School Medicine Box 357650 Seattle WA 98195-7650 tel. 206-685-3014 fax. 206-543-3480 email: dcoder@u.washington.edu ----- Original Message ----- From: Barsky, Lora <LBarsky@chla.usc.edu> To: cyto-inbox Sent: Tuesday, April 04, 2000 12:45 PM Subject: Attn: BD LSR users Hello, I found a couple of replies to the purdue site regarding the new BD LSR, but I'm hoping to hear from more individuals who have already gotten their BD LSRs up and running. Is the instrument easy to use, if you're not an "expert"? Could students easily operate the instrument? Is alignment easy to do with the calibration beads? And the optics, is it a matter of switching only bandpass filters or is there dicroic involvement? Does anyone have a 3-color LSR? And finally, what type of lab do you have it in, a Core, private lab, etc.? Thanks for the time, Lora Barsky
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