What beads are you using? I'm guessing you don't add PerCP if you use the BD
beads, 'cause the PerCPs are very unstable in my hands. If I mix all 4
(unlabeled, FITC,PE, PerCP) they are useless after about an hour. (After that I
no longer have single-color beads!) I have to make new dilutions each time. Do
you know something I don't?
David McFarland
Howard Hughes Medical Institute
Flow Cytometry Facility
Vanderbilt University Medical Center
"Jane S. Miller" <miller@medicine.tamu.edu> on 03/30/2000 01:23:34 PM
(Embedded image moved to file: pic08453.pcx)From:(Embedded image moved to
file: pic05728.pcx)"Jane S. Miller" <miller@medicine.tamu.edu> on 03/30/2000
01:23 PM
To: Cytometry Mailing List
<cytometry@flowcyt.cyto.purdue.edu>
cc: (bcc: David McFarland/VUMC/Vanderbilt)
Subject: Re: FACSComp QC
I run FACSComp each day that I run immunofluorescent samples. A mix of beads
will
keep a week or more. I don't make a new mix every time. Jane Miller
>>> Tami Rosario <trosario@cellmate.cb.uga.edu> 03/29/00 12:55PM >>>
I have a question for those of you who run FACSComp on the FACSCaliber
for QC. Do you run this each time you start up the cytometer or only
occasionally. I have been told that I should run FACSComp each day that
I run samples, but the beads are fairly expensive. Any alternatives?
Thanks,
Tami
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