Andrew, Under some circumstances, I have experienced non-specific staining with Annexin V-FITC which went away with Annexin V-PE. Lori ----- Original Message ----- From: Andrew Oberyszyn <oberyszyn.2@osu.edu> To: cyto-inbox Sent: Monday, March 27, 2000 2:48 PM Subject: Annexin V help > Hi everybody in Flow land, > I hope someone can steer me on this one. > I have a user who is looking at Annexin V- FITC/PI staining of Caco-2 > cells. The problem that I am encountering is that the untreated, Annexin > V- FITC only stained cells are a log higher than untreated/unstained cells > (see attached Powerpoint slide). I looked under a flourescent microscope > and saw considerable surface staining in the Annexin only samples. The > user is using the Pharmingen kit (5 ul of Annexin V). > I have not seen this happen (Annexin V so much higher than unstained) in > other cell lines that I've analyzed. Would it be "legal" to adjust the > volts to bring the Annexin V Only stained population into the negative > region or is it more correct to just set the gate as I've indicated on the > attached slide (marked with an *)? > Am I missing something obvious? Please help! > > Thanks in advance! > Andy > > ---------------------------------------------------------------------------- ---- > (:)-(:)-(:)-(:)-(:)-(:)-(:)-(:)-(:)-(:)-(:)-(:)-(:)-(:) > Andy Oberyszyn, M.S. > The Ohio State University > Analytical Cytometry Laboratory > 416 Comprehensive Cancer Center > 410 West 12th Avenue > Columbus, Ohio 43210 > Tel: 614/292-FLOW(3569) > Fax: 614/292-7335 > E-Mail: cytometry@osu.edu > Web Page: > <http://cytometry.med.ohio-state.edu>http://cytometry.med.ohio-state.edu > (-):(-):(-):(-):(-):(-):(-):(-):(-):(-):(-):(-):(-):(-) > > "What if the Hokey Pokey is really what it's all about?!?"
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:55:41 EST