Dear All, We have some large cells (70-80um) to sort on our new FACSVantage, and have no experience with cells of this size. Before we run through gallons of sheath, we would like to hear of your experience with large cell/large tip sorting. It looks like the 300um tip is recommended for this size cell. The Vantage is running well on normal 70um tip for blood and cultured cells. (Given the problems that occur with a new machine). All advice Welcome! Thanks Randall Smith Oregon Health Sciences University Flow Cytometry Core Facility Portland, Oregon
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