Dear Mara and other flowers, Within our institute we have several Cailburs, Facsscans a Star and a MoFlo and there is absolutely no influence due to the brand of sheat fluid you use. Since we have taken over the main production from Ortho we have also looked into the production of sheat fluid. We found that the most important item for this product is the consistency of the quality and the purity. Therefore we decided that you had better order this product at a company specialised in the production of huge volume buffers like Mallinckrodt Baker. Sheath fluid is one of the products on which B&D and Coulter is earning a lot of money. Purchasing sheat fluid from this source can easily degrease your cost on sheat fluid, taking in account the amount you use per year can make this very interesting. For more in formation contact clbreagents@clb.nl <mailto:clbreagents@clb.nl> Best regards, John John Voorn CLB Reagents Productmanager Plesmanlaan 125 j_voorn@clb.nl <mailto:j_voorn@clb.nl> 1066CX AMSTERDAM the Netherlands tel +31-(0)20 5123246 fax +31-(0)20 5123570 web site www.clb.nl <www.clb.nl> -----Original Message----- From: Mara Rocchi [SMTP:roccm@blackie.mri.sari.ac.uk] Sent: maandag 27 maart 2000 14:37 To: Cytometry Mailing List Subject: sheath fluid for Calibur Dear Flowers, we are in the process to buy a FACSCalibur, and we would like to here your suggestion abut the kind of sheath fluid we must/can use. We aren't planning any sorting, maybe some DNA work, lot and lot of phenotyping and intracellular staining. From the archives looks like that different people use different solutions, and for different reasons. I have been using sterile saline for FACScan and FACStar, and D'PBS for FACSort, but being new to the Calibur world, I would like to be sure that those alternatives to the BD sheath fluid are OK. Thanks a million in advance Mara Mara S.L. Rocchi Moredun Research Institute Penicuik EH26 0PZ Scotland, UK e-mail: roccm@mf.mri.sari.ac.uk
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