RE: non-sterile cell strainers

From: Barber, Lesley (PMC) (l.barber@pmci.unimelb.edu.au)
Date: Thu Mar 23 2000 - 17:56:42 EST


I replied to writer but repeat to list. I had my tech service people make
metal strainers using fine metal mesh soldered to a metal handle, similar to
flat tea strainers. Different sizes can easily be made so that even large
organs can be processed. These can be washed and sterilized between use. I
have some that have been in continual use for the last twenty years, very
cost affective!
Cheers Lesley
Mrs. Lesley Barber
Edith Margaret Collie Centre for Blood Cell Therapies
Div. Haematology/Medical Oncology
Peter MacCallum Cancer Institute
East Melbourne, 3002
VICTORIA , AUSTRALIA
Tel 61 3  9656 1957
Fax 61 3 9656 1811


> -----Original Message-----
> From: Joseph Webster [SMTP:J.Webster@centenary.usyd.edu.AU]
> Sent: Thursday, March 23, 2000 2:37 PM
> To:	Cytometry Mailing List
> Subject:	Re: non-sterile cell strainers
>
>
> Lots of people here just cut small bits of nylon mesh for
> this purpose; lay (or hold) over the test tube and pipette
> your cell suspension through.
>
> Someone else may care to post (again) your favourite mesh
> supplier...
>
> :-) Joseph. :-)
>
> At 00:36 18/03/2000, Tessa Kerre wrote:
> >Dear flowers,
> >In the lab we are preparing murine organ cell suspensions, and therefore
> >we need a high amount of cell strainers. Now we use individually packed
> >sterile cell strainers, while we would be satisfied with non-sterile
> >cell strainers in bulk.
> >
> >I have asked some companies including BD, Nunc, etc, but none seem to
> >have this.
> >
> >Does anyone know of a company who would supply this?
> >Or do you have other suggestions?
> >Thanks,
> >Kind regards,
> >Tessa



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