At a recent flow cytometry workshop in the Research Triangle Park area the following question was asked: Is it possible to measure viability in a cell population and then fix the cells prior to analysis by flow cytometry. Can the dye stay in the dead cells without leaking into the live cells? I remember that Dr. Allan Pollock worked on these techniques in the 1980's . Does any one have any knowledge or information on this type of protocol. The research groupe wants to apply this technique on AIDS blood. Thanks Bob Robert M. Zucker, Ph.D U.S. Environmental Protection Agency National Health and Environmental Effects Research Laboratory 2525 NC Highway 54, MD 72 Research Triangle Park, North Carolina, 27709 tel: 919-541-1585 fax 919-541-4017 e-mail: zucker.robert@epa.gov
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