Hello Cliff, We have looked at a number of cell types to try and address these questions on the MoFlo. Others have examined this also using different instrumentation. We had a poster on viability at ISAC in 1998 (Cytometry Suppl 9, page 122 ), and will have a follow-up poster at ISAC in May. Initially we looked at viability by PI after sorting, as well as performance in cell culture. We have not yet looked at the more specific functional characteristics that you mentioned, although I think others have... (see Chris Groves, Cytometry Suppl 9, page 137). I don't think it's too late to try and organize an informal get together at ISAC for those interested in sharing their experience with cell "quality of viability" after high speed sorting. Please let me know if you're interested. I know Dave Coder and Ger van den Engh will have a tutorial on high speed sorting at ISAC, and may also address these issues. One other resource for you to try is the MoFlo Users Group, since there you will find folks who have been doing this type of thing for quite some time, and they may have more specific information related to the cells you're using. Peter > ---------- > From: Cliff McArthur[SMTP:cytocliff@netscape.net] > Sent: Monday, March 06, 2000 10:16 AM > To: Cytometry Mailing List > Subject: HOW viable after high-speed sorting? > > > Hi there, flow aficionados. > > I know we've all addressed the post-sort viability (after high-speed > sorting) > question many times over. What I am looking for is not "whether" but "how > much," so to speak. To that end, I'd like to ask the List if anyone knows > of > any published work that has addressed the question not of whether or not > cells > are viable after such sorting (we know they are, or can be) but "how" > viable, > that is, do they produce less cytokine or other product, proliferate a > little > more slowly, or survive for less time, etc., etc. under the same > conditions > versus "low-speed" sorted cells? Shucks, references that just address > this > question, if it is not the primary objective of the work, would be great > to > have. > > If anyone is willing to share their unpublished insights, I'd love to hear > them as well. > > Thank you very much, > Cliff McArthur > University of California at San Francisco > Departments of Medicine and Immunology/Microbiology > 415-502-6860 > > ____________________________________________________________________ > Get your own FREE, personal Netscape WebMail account today at > http://webmail.netscape.com. >
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