We sort bacteria regularly using a Vantage. We generally are not able to see them above the noise level on forward scatter, so we generally trigger on side scatter, with the side scatter amp in log mode. This works well, although one must carefully adjust the fluorescence obscuration bar to minimize drop drive noise in the side scatter channel. We have not done this with the higher speed modifications - if the drop drive noise is not a problem it should work in these circumstances as well. Marty Bigos Stanford Shared FACS Facility >Dear Flowers, > >I will be needing to sort bacteria soon. I've never sorted bacteria before >so any hints, suggestions, >advice are welcome. Also, could someone recommend alignment beads for >bacterial sorting. My >instrument is a FacsVantage. Thanks in advance for your help. > >Jackie Saleh >Aventis Pharmaceuticals
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