Paul,
We've worked pretty extensively with cynos and have used both FACS Lysing
Solution and the various Prepstations and reagents from Coulter (Q-Prep,
TQ-Prep) with good results from both. We've also done gradient separations
on cyno blood with fairly good separation, although in our hands, the
procedure is much more time consuming (need to spin longer than human
cells), the preps aren't always that clean (RBCs and granulocytes mixed in
with the mononuclear cells) and I'm not convinced that the preps are any
better than lysed samples.
As for antibodies, check with Pharmingen and Coulter for cross-reactive
clones.
Good luck,
Patrick B. Lappin, D.V.M., Ph.D.
Diplomate, American College of Veterinary Pathologists
Sierra Biomedical, Inc.
587 Dunn Circle
Sparks, NV 89431
775-331-2201 (Phone)
775-331-2289 (Fax)
-----Original Message-----
From: Paul Kuon [SMTP:pmk3351@louisiana.edu]
Sent: Monday, February 28, 2000 8:55 AM
To: Cytometry Mailing List
Subject: Cynomolgus testng
Anyone out there had any experience working with Cynomolgus monkeys?
Specifically a whole blood lysing procedure which gives better population
separation.
Thank you
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