The honorable Mr. Roederer wrote: For those interested in more amplification than the SA-biotin system (or rather, interested in more fluorescence signal per antigen), you might want to consider a system devised by Peter Lansdorp (at the Terry Fox Cancer Center in Vancouver). Molecular Probes also does a similar thing with its AlexaFluor 488 and FITC. Antibody specific for your Ag conjugated to FITC Rabbit anti-FITC Ab conjugated to AlexaFluor 488 AlexaFluor 488 Goat anti-rabbit (Alexa has a similar emission to FITC.) Regina Regina H. Harley, M.S. Lab Supervisor Flow Cytometry Facility Center for Vaccine Development HSF 456 685 West Baltimore Street Baltimore, MD 21201 (410) 706-7376 FAX: (410) 706-6205 rharley@medicine.umaryland.edu
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