To everybody, I'm working with Kluyveromyces lactis, a yeast very similar to S.cerevisiae. I'm studing the feasibility of screening, by FACS analysis, for multicopy suppressors of a mutation that blocks the cells with a doubled DNA content. To this end I need a protocol to stain DNA of live cells in such a fashion that will let me sort and collect transformants that have N DNA content. If somebody can give me any help, it will be very much appreciated and acknowledged Thanks in advance, Daniela -- Daniela Uccelletti, PhD Dpt. Molecular and Cell Biology Boston University 700 Albany Street W216 Boston MA phone: 617-414-1055 e-mail: danielau@bu.edu
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