Why do you have no Calcium in your media? Are you specifically looking for mobilization from intracellular stores? Such a flux is smaller and faster. Are your cells loaded OK, how does the brightness compare to unlabelled cells? Did you try with ionomycin. Add 1mM Ca++ and it'll probably work, everything else looks OK. You can't get no flux if you ain't got no calcium Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352
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