I wonder if anyone has experience with Caltag's goat anti-rat Ig-PE conjugate used as a second antibody for intracelluar staining in mouse cells? I see that most relevant publications use Pharmingen abs. Is there a good reason? Caltag's product works fine for cell surface staining - does it not get into my saponin-permeabilized cells? Thanks for any suggestions. Barbara Replies to barbaraJ@qimr.edu.au ************************************************************************** * Grace Chojnowski, * * Flow Cytometry & Confocal Microscopy Laboratory. * * Queensland Institute of Medical Research * * The Bancroft Centre Tel: 61-7-3362 0314 * * 300 Herston Road Herston QLD 4006 Tel: 61-7-3362 0315(Voicemail)* * AUSTRALIA Email:graceC@qimr.edu.au Fax: 61-7-3362 0107 * **************************************************************************
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