Hello everyone. I have a client that wants to do analysis of RBCs. This is new to me. Seems everyone else wants to make sure we avoid the RBCs! Basically, they are injecting mice with a FITC-labeled ligand that should only be bound (and internalized?) by cells that express the receptor for said ligand. They have control mice, and transgenics that express the receptor. The question is where is it expressed? We did some earlier experiments excluding the RBCs, but now they want to look at them as well. They are looking to me for advice and I don't have any to give them. So, does anyone have some good advice to pass along? I'll even give you the credit! David McFarland Howard Hughes Medical Institute Flow Cytometry Facility Vanderbilt University Medical Center
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