Lee Anne, Just a few thoughts on this topic - which are mostly anecdotal but probably reliable :-) EDTA is not an ideal (read "suitable") anticoagulant for storage of samples, particularly if you are doing a whole blood lysis technique - can you ask for Heparin instead (or as well if you want the EDTA for cell counts)? Even in Heparin the max storage should be 48 hours - the Friday to Monday interval is pushing things a little. Whenever storing samples we do so at room temperature, in their native plasma (in the original collection container), they seem to like this the best (for whole blood lysis preparation up to 48 hours); it is sterile and well buffered. I'm interested in the thoughts of the rest of the group ... Peter Chapple Melbourne AUSTRALIA >I have a basic question. I am receiving Rat and Dog blood in 3 ml vacutainer >tubes with EDTA. We are looking at basic cell surface markers. If I >receive blood on a Friday, how can I preserve the blood to analyze on >Monday? Can I leave it in the tube it was drawn in? Should I take it out >of the vacutainer and preserve it another way? Should I stain and fix it >for later analysis? And would preservation be best at room temp or 4*C? I >have read some literature on this subject but have not found my exact >situation, or what I think is my situation. > >If any one would know, you all would. Thanks in advance. Any suggestions >would be helpful. >Respectfully, >Lee Anne Talbot >Scientist >Boehringer Ingelheim Pharmaceuticals >Ridgefield, CT >USA >203-798-4133
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