> > I'm sure you know this already, but I think it's worth mentioning that >fluorescein contributes to the fluorescence inside the cell but you >add/incubate the cells with the non fluorescent >5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester [5(6)-CFDA,SE >according to Molecular Probes], and not CarboxyFluorescein Succinimidyl >Ester as might be thought of with the nomenclature CFSE. I am >embarrassed to admit that I was fooled by this on my first unsuccessful >experiment using this method. > Yes the abbreviations are confusing. When Lyons and Parish first published their paper on using CFSE to track cell division, Molecular Probes was using the abbreviation CFSE to refer to 5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester. (Lyons, A. B., et al. (1994) Determination of lymphocyte division by flow cytometry. Journal of Immunological Methods 171:131-137.). Since this is THE seminal paper on this method the abbreviation has stuck... A couple of years ago (I'm not sure exaclty when but I could check back through our Molecular Probes catalogs) they changed the abbreviation to 5(6)-CFDA,SE (or just CFDA, SE). Hence the confusion. Adrian PS the catalog number has stayed the same, ie C-1157
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