Calman,
Just last week, we performed a cross titration experiment with 53-6.7 and
CT-CD8a (from Caltag) and found no detectable competition between the two
antibodies.
John
on 1/24/2000 6:06 PM, Calman Prussin at CPRUSSIN@niaid.nih.gov wrote:
>
> A collaborator of mine has performed an in vivo depletion of CD8 cells in
> mice using clone 2.43. When he went to look for CD8 cells using PharMingen's
> 53-6.7 mAb they appeared depleted. Unfortunately, when we tried a simple
> blocking experiment in vitro, pre-incubation with 2.43 blocked subsequent
> 53-6.7 binding, suggesting that the "depletion" may simply be due to
> antibody cross-competition (the 2 mAbs recognize the same or closely related
> epitopes).
>
> Does anyone in the group know of other commercially available CD8alpha
> antibodies that do not cross compete with either 2.43 or 53-6.7? We cannot
> use CD8beta antibodies.
>
> Is there a mouse CD data base as exists for human CD antibodies? Thanks.
>> _______________________
>> Calman Prussin
>> Laboratory of Allergic Diseases
>> NIAID/ National Institutes of Health
>> 10 Center Dr., MSC-1881
>> Bethesda, MD 20892-1881
>> (301) 496-1306 (Voice)
>> (301) 480-8384 (Fax)
>> calman@nih.gov
>>
>
***************************************************************************
John D. Altman, Ph.D.
Emory University Vaccine Center at Yerkes
954 Gatewood Road
Atlanta, GA 30329
Office: (404) 727-5981
FAX: (404) 727-9005
FAX2: (404) 727-8199
Lab: (404) 727-8914
email: altman@microbio.emory.edu
***************************************************************************
Tetramer Core Facility:
http://www.niaid.nih.gov/reposit/tetramer/index.html
***************************************************************************
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