Hello, We would like to enumerate malaria infected reticulocytes and normocytes by flow cytometry (murine malarias). Currently we count infected RBC using thiazole orange staining, and can generally distinguish retics from infected cells by the level of staining, but obviously cannot tell infected retics from infected normocytes. We have a Coulter XL, so are somewhat limited in the dyes that we can use. Can anyone suggest a DNA-only and an RNA-only dye that could be used simultaneously for this kind of analysis? Thanks in advance for your help. Anne ==================================== Anne Avery, VMD, PHD Department of Pathology Colorado State Univ Ft. Collins, CO 80523-1671, USA voice:(970)491-1170 fax:(970)491-0603 aavery@cvmbs.colostate.edu ====================================
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